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1. Drink 8 10 glasses of water a day. Drinking less fluid will not cure water retention and can actually make it worse. Following electroporation the cells were rested in X VIVO 15 medium (BioWhittaker) containing HABS, GM CSF and IL 4 as above at 37C in humidified 5% CO2 for one hour. Subsequently the cells were washed once and suspended in the maturation medium containing 1100 IU/mL TNF a and 1.0 g/mL prostaglandin E2 for two more days. MDCs were collected and assayed for compliance with release criteria..
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